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KMID : 1101320080400010048
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Korean Journal of Clinical Laboratory Science
2008 Volume.40 No. 1 p.48 ~ p.54
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Virulence Genes of Staphylococcus aureus Isolated in Daegu and Gyeongsangbuk-do Areas
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Jeon Seok-Jae
Lee Hee-Moo
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Abstract
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Nine types of staphylococcal enterotoxin (SE) genes (sea~see, seg~sej), 3 types of virulence genes (eta, etb, tst), mecA and 16S rRNA as internal positive control were detected from 187 clinical MRSA (methicillin resistance Staphylococcus aureus) strains isolated from a variety hospitalized patients in Daegu and Gyeongsangbuk-do areas using the multiplex PCR. The frequency of the S. aureus strains harboring recently reported SE genes (seg~sej) were found to be very high (65.9%) and greater than that of the strains harboring classical SE (sea~see) genes (47.8%) as previously established. Taking into account that the newly described pairs form SE genes (i.e., sec+seg+sei, seg+sei) were many, in the other hand, single form SE genes (i.e., seg, seh, sei and sej) were rarely detected. The S. aureus with pairs form enterotoxigenic genes become more potentially toxigenic strains. Furthermore, this work indicated a systematic association between the seg and sei genes and their high incidence among the S, aureus strains, which suggests that these two SE’s could be an important phylogenetic link among the staphylococcal enterotoxins.
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KEYWORD
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Staphylococcus aureus, Virulence genes, Enterotoxin, PCR
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